The Giallorossi secured a 2-0 win over Fiorentina last time out, thanks to a Paulo Dybala brace, and are just three points off the Champions League qualification spots.
Spezia, meanwhile, sit in 15th place but have pulled away from the relegation zone and will be optimistic that they can avoid the drop, with the scalp of Roma a welcome boost in their fight to remain in the top flight.
Spezia v AS Roma: Predicted line-ups
Roma coach Jose Mourinho remains without midfielder Georginio Wijnaldum, as the Dutchman closes in on a return from a broken leg, whilst Nicola Zalewski may be rested after being withdrawn against Fiorentina.
Spezia will be without the suspended Dimitris Nikolaou, whilst Jeroen Zoet misses out through injury.
Spezia predicted XI v AS Roma (3-5-2): Dragowski; Amian, Kiwior, Caldara; Holm, Bourabia, Ampadu, Bastoni, Reca; Gyasi, Nzola
AS Roma predicted XI v Spezia (3-4-2-1): Rui Patricio; Mancini, Smalling, Ibanez; Celik, Cristante, Matic, Spinazzola; Pellegrini, Dybala; Abraham
Spezia v AS Roma: When does the Serie A fixture start?
Kick-off in the Serie A Matchday 19 fixture of Spezia v AS Roma is set for 18:00 CET, local time in Italy, on Sunday 22 January.
For viewers in the United Kingdom and Ireland, the game will start at 17:00 GMT. That is 12:00 ET / 09:45 PT in the United States.
Spezia v AS Roma: Where to watch the Serie A fixture?
Spezia v AS Roma in Serie A will be shown on BT Sport 1 in the United Kingdom and Ireland on Sunday 22 January.
For fans in the United States, Spezia v AS Roma will be on Paramount+ along with other key fixtures in Serie A Matchday 19.